Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD 4FC4 Chain:I ((3-250)) ----------------------TDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL---DPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALCNWLVCLAIWMAIRTE-GTAKFLAIWWCLLAFIASGYEHSVANMTLFALSWFGHHS--DAYTLAGIGHNLLWVTLGNTLSGVVFMGLGYWYATP-------

General information: TITO was launched using: RESULT: Template: 4FC4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 1309 -224116 -171.21 -903.69 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain I : 0.77 3D Compatibility (PKB) : -171.21 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4FC4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FC4-query.scw PDB file : Tito_Scwrl_4FC4.pdb: