Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKFLDMSLVQRIVVGILVGILFGIF---FPTWTFISILGTLFVSCLKAIAPILVFFLTMASIAKH-KIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAF-----------RARSESTKELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIVGIGFIIG--VIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDCLDGLSER 4OYF Chain:A ((9-416)) --EYPVLQKILIGLILGAIVGLILGHYGYAHAVHTYVKPFGDLFVRLLKMLVMPIVFASLVVGAASISPARLGRVGVKIVVYYLLTSAFAVTLGIIMARLFNPGAGIHLAVGGQ-------LVHILLDIVPTNPFGALANGQVLPTIFFAIILGIAITYLMNSENEKVRKSAETLLDAINGLAEAMYKIVNGVMQYAPIGVFALIAYVMAEQGVHVVGELAKVTAAVYVGLTLQILLVY-FVLLKIYGIDPISFIKHA-KDAMLTAFVTRSSSGTLPVTMRVAKEMGISEGIYSFTLPLGATINMDGTALYQGVCTFFIANALGSHLTVGQQLTIVLTAVLASIGTAGVPGAGAIMLAMVLHSVGLPLTDPNVAAAYAMILGIDAILDMGRTMVNVTGDLTGTAIVAKTE--------------------

General information: TITO was launched using: RESULT: Template: 4OYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1841 -256803 -139.49 -672.26 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -139.49 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_4OYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OYF-query.scw PDB file : Tito_Scwrl_4OYF.pdb: