Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQILS 1PDN Chain:C ((16-59)) ---------------------------------------------------------------------------------------------RPLPNNIRLKIVEMAADGIRPCVISRQLR------VSHGCVSKILNRYQE---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1PDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 116 -32086 -276.60 -729.22 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain C : 0.56 3D Compatibility (PKB) : -276.60 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.773

(partial model without unconserved sides chains): PDB file : Tito_1PDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PDN-query.scw PDB file : Tito_Scwrl_1PDN.pdb: