Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQFQGSAKMKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEK---QKYELDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF 1TDP Chain:A ((7-85)) ------------TLYLNLSEAYKDP---EVKANEFLSKLVVQCAGKLTASNSEN---SYIEVISLLSRGISSYYLSHKRIIPSSMLTIYTQIQKDIK------------------

General information: TITO was launched using: RESULT: Template: 1TDP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 -13914 -50.05 -183.07 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -50.05 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_1TDP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TDP-query.scw PDB file : Tito_Scwrl_1TDP.pdb: