Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMAIFYQDMTNEEITNLTLAIANSGDVIDLS-SLEGIKVDKHSTGGVGDTTTLILAPLVASVGVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPADKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLAKTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALGSQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAI---------VEHPERILTARYTMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGESLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE 2WK6 Chain:B ((35-448)) -QLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALS----------------------------

General information: TITO was launched using: RESULT: Template: 2WK6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2501 -35588 -14.23 -88.09 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -14.23 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_2WK6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WK6-query.scw PDB file : Tito_Scwrl_2WK6.pdb: