Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGK-KKCAHFAHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLAIEIQCSRLSHQRFIERTQNYLNNS--YQVWWILGNSFLGQSQF--SLIE--KSCCYYNRKRGVHCWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEKVSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLYRMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK 2V9P Chain:A ((1-279)) -TLNESKFDFGTMVQWAYDHKYAEESKIAYEYALAAGSDSNARAFLATNS-----QAKHVKDCATMVRHYLRAETQALSMPAYIKARCKLATGEGSWKSILTFFNYQ--NIELITFINALKLWLKGIPKKNCLAFIGPPNTGKSMLCNSLIHFLGGSVLSFANHKSHFWLASLADTRAALVDDAT----------HACWRYFDTYLRNALDGYPVSIDR---------------------KHKAAVQI------KAPPLLVTSNIDVQAEDRYLYLHSRVQTFR--FEQPCTDEQPFNIT--------------------DADWKSFFVRLWGRLDLID

General information: TITO was launched using: RESULT: Template: 2V9P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1135 -25502 -22.47 -96.23 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -22.47 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_2V9P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V9P-query.scw PDB file : Tito_Scwrl_2V9P.pdb: