Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFENKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPEYANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLPKQIPVSGMVERLP-VD-HPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVYYDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYK-NEPLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVG-TADAFHFIIEDPVAPWNNGVFALTWDEQGQVRVLN---EPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLERIIPNMEAYFSDYF 2HV2 Chain:A ((5-400)) -------TTKRVKKMGKEEMKEMFDLVIYAFNQEPTAER-------QERFE---KLLSHTQSYGFLIDEQLTSQVMATPFQVNFHGVRYPMAGIGYVASYPEYRGEGGISAIMKEMLADLAKQKVALSYLAPFSYPFYRQYGYEQTFEQAEYTIKTEDWPRVKRVPGTIKRVSWADGKEVIKDVYLENQRAHSGGVIRETWWLDYTLNRASKPNNQAIYY-SSEGKAEGYVIYRIAAGTFEIVEWNYLTNTAFKALAGFIGSHSGSVQSFHWINGFAGKDLNDLMPTPAASVKILPYMMARIVELQTFLEKYPFQSGEKETYSLEIEDSYGPWNEGIWTITIDEQGKATVTKGAA---TAALKADIQTWTQLFLGYRSAETLSFYERLQGDATIAQRLGQRLVKGMPILEDYF

General information: TITO was launched using: RESULT: Template: 2HV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1953 -97339 -49.84 -252.83 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -49.84 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_2HV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HV2-query.scw PDB file : Tito_Scwrl_2HV2.pdb: