Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQEDVKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLG-EERKWVHYGLTSTDVVDTAYGYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDLHAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSENMAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNLTVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTRE--EAYDLVQPKTAYAWDNQVQFRPLLEED--PTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE 4EEI Chain:B ((1-430)) MIKRYDVAEISKIWADENKYAKMLEVELAILEALED-RMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQFTAETGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEIRHLHRSDVFEVYEGF------------KKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVA--NTPFMREDCYKIVQQVAFDL----ESFSKKLQKVMHDEHNIILDIPEMDFEGIKKTYLKEIDHVFDRSV--

General information: TITO was launched using: RESULT: Template: 4EEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1899 -28915 -15.23 -70.52 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -15.23 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4EEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EEI-query.scw PDB file : Tito_Scwrl_4EEI.pdb: