Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIIRVKNAEEGGKKAFEIIKEGMNKGAK------VLGLATGSTPVTLYKEMT----SSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKK--PFKETFVPDGKAEDLEAACKKYDQIIDAH-PIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRFFDK-AEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNHDNVVVIIDEEAASKL 1HOT Chain:B ((1-247)) MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGTPMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAENINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIKTLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEGCVNHMWTISCLQLHPKAIMVCDEPSTMEL

General information: TITO was launched using: RESULT: Template: 1HOT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1233 46817 37.97 200.93 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : 37.97 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_1HOT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HOT-query.scw PDB file : Tito_Scwrl_1HOT.pdb: