Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDTSKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARFAAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAY-VPQNRERIFLIGHSRDACTEQV-FPIIGSSPTS-DQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGDEGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQEVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQGFPDWAFDRASQVN-SDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL 1FJX Chain:A ((12-318)) LRFIDLFAGLGGFRLALESCGAECVYSNEWDKYAQEVYEMNFGEKPE---GDITQVNEKTIPDH---DILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIPQKRERIYMICFRNDLNIQNFQFP----KPFELNTFVKDLLLPDSEVEHL---VIDRKDLVMT---NQEIEQTTPK----TVRLGIVGKGGQGERIYSTRGIAIGLSAYGGGIF------------AKTGGYL----VNGKTRK----------------------------LHPRECARVMGYPD-----SYKVHPSTSQAYKQFGNSVVINVLQYIAYNI--------

General information: TITO was launched using: RESULT: Template: 1FJX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1723 -92932 -53.94 -306.71 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -53.94 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_1FJX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FJX-query.scw PDB file : Tito_Scwrl_1FJX.pdb: