Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTSGFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEV--PRDEKRGQFARASWGIDYHDILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQ--VDTGELIDVAVAQRAGLGFIGRNGLLITEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSYQTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPMLSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLT-DVRPVIRGTAAWAIGRLGTKEPEKWLDVLDEILAKET-EEVVINELEHAQKLLRRKLK 5D08 Chain:B ((32-404)) YQLKEELIEYAKSIGVDKIGFTTADTFDSLKDRLILQESLGYLSGFEEPDIEKRVTPKLLLPKAKSIVAIALAYPSRMKDAPRSTRTERRGIFCRASWGKDYHDVLREKLDLLEDFLKS--K------HEDIRTKSMVDTGELSDRAVAERAGIGFSAKNCMITTPEYGSYVYLAEMITNIPFEPDVPIEDMCGSCTKCLDACPTGALVNPGQLNAQRCISFLTQTKGFLPDEFRTKIGNRLYGCDTCQTVCPLNKGKDFHLHPEMEPDPEIAKPLLKPLLAISNREFKEKFGHVSGSWRGKKPIQRNAILALAHFKDASALPELTELMHKDPRPVIRGTAAWAIGKIGDPA---YAEELEK-ALEKEKDEEAKLEIEKGIELLK----

General information: TITO was launched using: RESULT: Template: 5D08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1935 21568 11.15 58.77 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : 11.15 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_5D08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D08-query.scw PDB file : Tito_Scwrl_5D08.pdb: