Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPASQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKHIDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRAVVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEFVKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKSAEAFGAGE 3NGL Chain:C ((2-270)) --KILRGEEIAEKKAENLHGIIER---SGLEPSLKLIQIGDNEAASIYARAKIRRGKKIGIAVDLEKY-DDISMKDLLKRIDDLAKDPQINGIMIENPLPKGFDYYEIVRNIPYYKDVDALSPYNQGLIALNREFLVPATPRAVIDIMDYYGYHE--NTVTIVNRSPVVGRPLSMMLLNRNYTVSVCHSKTKDIGSMTRSSKIVVVAVGRPGFLNREMVTPGSVVIDVGINY-VNDKVVGDANFEDLSEYVEAITPVPGGVGPITATNILENVVKAAE------

General information: TITO was launched using: RESULT: Template: 3NGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1450 -186160 -128.39 -692.04 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -128.39 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.628

(partial model without unconserved sides chains): PDB file : Tito_3NGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NGL-query.scw PDB file : Tito_Scwrl_3NGL.pdb: