Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GKNGLKDCQVSITATSEARR---SFWDTQYADYNDD-------VEISAGRHKFVIHVEQDC------GGSKVSGTIPPHHLVILSPMSGGGRRTIINSA-------GEVRLAAT---- 1F3U Chain:A ((2-119)) AERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPA

General information: TITO was launched using: RESULT: Template: 1F3U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 12843 72.15 141.13 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 72.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_1F3U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F3U-query.scw PDB file : Tito_Scwrl_1F3U.pdb: