Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKEKMFNRGILVASHGN-FASGALMTAEMFVGETTNDRVRTLGLMPGENIVEFEHYFKNQVNELLD--SNQEVIVLTDLIGGSPNNVALSRFLN-LDSVDIVTGFNIPLLVELISSYDSKINLEE----IVHNAQNSLFNVKQQLNVEEEEDLCL 3GDW Chain:B ((2-135)) -----NANVGVFVLMHGDSTASSMLKTAQELLGT--S-IGTAMNMPLTMEVQTMYEQLRNQVITQ-KESLNNGILLLTDM--GSLNSFGNMLFEETGIRTKAITMTSTMIVLEAIRMASVGRSLEDIYQNIQLSFESVVREQFRS-----------

General information: TITO was launched using: RESULT: Template: 3GDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 601 -30627 -50.96 -243.07 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -50.96 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_3GDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GDW-query.scw PDB file : Tito_Scwrl_3GDW.pdb: