Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIEQLQSKIEQARTLYKEFRLYDLGSFALNRFTPKDGFEQVLNVRYGLKPRHRLDIYRSTKRLAHRPLIVFVHGGAWQHGNKRDYLFIGEAFTKE-GYDVAVINYQLAPKNIFPSYVDDLTQALNYLHQNQEKLEISTENIVLMGHSAGAFNVMSAVYHPKPNTIQCLGNIKAIFGLAGPYHFDYKGDP-LA-----------EDAFDQSI--SYK-EVMP----YY-FVNQNHIKHYLLMAENDQLVKKENTFDLDQALRQSGNHSHIAVIPKTGHITILASLSSFVSHYFRTKRTILHFLDEVF 3GA7 Chain:A ((74-321)) ----------------------------------------------------DVTTRLYSPQP--TSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIGFAGDSAGAMLALASALWLRDKHIRC-GNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWYCLFNNDLTRDVPPCFIASAEFDPLI-DD-SRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRM--MTIADDALQDGARFFMAR-

General information: TITO was launched using: RESULT: Template: 3GA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1275 -61478 -48.22 -270.83 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -48.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3GA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GA7-query.scw PDB file : Tito_Scwrl_3GA7.pdb: