Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFQLGEDHDSGMNEMNLIPLIDIMLVLMIIFLVTATVANPSIPLTLPKTTAEIID--PPPKAITISINANGEVAW--DTQV---ISLDELQKRFQEAGQGDVKPTVQLRADKESKYDTVAQVMSRASEAGLSDIAFVSEN 5BY4 Chain:A ((5-109)) ------------------------------------PIITQSVEVDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKANPKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQP

General information: TITO was launched using: RESULT: Template: 5BY4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 335 18888 56.38 192.73 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 56.38 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_5BY4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BY4-query.scw PDB file : Tito_Scwrl_5BY4.pdb: