Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLAAFEAFVKVMETGSISVAADQLFITQPAVTKRIHSLEEYFGVKLFESAGRGVQATHAAHSLLPKVKSWLNELRDIHHTLSHEQTQIQGRLKIGTSHHIGLHHLPAPLKHFVQQFPQVTLDVHFVDSEQAHEQVLAGDLELAFLTLPPSGDERLNYITIWNDPLVFVAAPFHPLAQKKNLILQDLIEYPSLLPAAQT--YTAQITLAEFEKQCLKPKITMSNNPLESIRMLVSIGLGWSVLPKTLLN---QDMQQLDLN-VEMNRQLGMVWHPARTQSRAMQELIKMMQE 1IZ1 Chain:B ((1-289)) MEFRQLKYFIAVAEAGNMAAAAKRLHVSQPPITRQMQALEADLGVVLLERSHRGIELTAAGHAFLEDARRILELAGRSGDRSRAAARGDVGELSVAYFGTPIYRSLPLLLRAFLTSTPTATVSLTHMTKDEQVEGLLAGTIHVGFSRFF-PRHPGIEIVNIAQEDLYLAVHRSQSGKFGKTCKLADLRAVELTLFPRGGRPSFADEVIGLFKHAGIEPRIARVVEDATAALALTMAGAASSIVPASVAAIRWPDIAFARIVGTRVKVPISCIFRKEK-QPPILARFVEHVR-

General information: TITO was launched using: RESULT: Template: 1IZ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1297 -96155 -74.14 -339.77 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -74.14 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_1IZ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IZ1-query.scw PDB file : Tito_Scwrl_1IZ1.pdb: