Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSTQQWQSVTWFEVDEHQAGQRIDNFLFSRLKGVPKSRIYRLIREGQVRVNKKRIKAETKLAIGDQIRVAPIRYEQKDETAAPVSDSVAQGLLSRVVYEDEGLLVVNKPSGIAVHGGSGVA--YGLIEALRAATGKKYLELIHRIDRDTSGLVMISKKRSTLKLLQDMLREHKIRKTYAAIVKGQVSLDKQLIDAPLFRYELANGERRVRV-SKEGKPSKTEWVVAERF-KNATLVHASPLSGRTHQIRVHGLSIGHPLVGDDKYGHNTAYTGPEARRLCLHAMRLDIPGYP-----TIEAPLPEDMTQLLEALRVAK 2I82 Chain:A ((7-216)) -----------------------------------------------------------------------------------------PQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRD--YPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPSPAEGLVDLPLICDWP-NRPK-QKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGRSHQLRVHMLALGHPILGDRFYA-SPEA-RAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD--------------

General information: TITO was launched using: RESULT: Template: 2I82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1024 -719 -0.70 -3.58 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -0.70 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2I82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I82-query.scw PDB file : Tito_Scwrl_2I82.pdb: