Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGPDMQAIILDTETHTLNG--L-PIEIAYAPIEINAGKLTL-----------DKSKLFDQLYQVGTPISYAAMAVHHILESDLENQP---HYKTF----KLPDT----TTYIIGHNI-DYDIAAIARC----GVDVS-HIKPICTLALTRKTW---PDAEAHNISALIY-MISQGSSKARELLKGAHRADADIILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALESAF 1Y97 Chain:A ((5-208)) ---PRAETFVFLDLEATGLPSVEPEIAELSLFAVHRS----SLENPEHGALVLPRVLDKLTLCMCPERPFTAKASEITGLSSEGLARCRKAGFDGAVVRTLQAFLSRQAGPICLVAHNGFDYDFPLLCAELRRLGARLPRDTVCLDTLPALRGLDRAH----GYSLGSLFHRYFRAE-------PS---SAEGDVHTLLLIFLHRAAEL------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Y97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 630 -29809 -47.32 -194.83 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -47.32 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_1Y97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y97-query.scw PDB file : Tito_Scwrl_1Y97.pdb: