Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRCNVMKILIIDNYDSFTFNLYQLLGEILISLKSRKEISDFEIVVKRNNEISLDEIRKLEFDRIIISPGPGSPDDERYFGISKQ-VILQLGKEIPILGVCLGMQGIAYCFGGKVIRAKLPMHGKISTINH-SDSGVFSGIPQDVEVMRYHSLIVDSSTLPECFIVTARVNSAYSDSEEEIMGIKHQTYPIEGVQFHPESFASEAGKTLLQNFVHSKL 1WL8 Chain:A ((1-186)) -----MMIVIMDNGGQYVHRIWRTLRYLG-----------VETKIIPNTTP-LEEIKAMNPKGIIFSGGPSLENTG----NCEKVLEHYDEFNVPILGICLGHQLIAKFFGGKVGRGEKAEYSLV-EIEIIDE--IFKGLPKRLKVWESHMDEVKE--LPPKFKILARS------ETCPIEAMKHEELPIYGVQFHPEVAHTEKGEEILRNFAKLC-

General information: TITO was launched using: RESULT: Template: 1WL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1078 -30671 -28.45 -168.52 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -28.45 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_1WL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WL8-query.scw PDB file : Tito_Scwrl_1WL8.pdb: