Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPLRLVSELDTALEFFIAKNAKGIVSISETEHSPMWSNTLPENGC---MSDFIRPEVQGKRSQDLPKFFRLNGSIYIYETLCLLEQSKIFFNENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 1QWJ Chain:A ((2-223)) ----PHLAALVLARGGSKGIPLKNIKRLAGVPLIGWVLRAALDAGVFQSVWVSTDHDEIENVAKQFGAQV-HRRSSETSKDSSTSLDAIVEFLNYH---NEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRH-Q--FRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMG-Y-LQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRF---

General information: TITO was launched using: RESULT: Template: 1QWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1087 -68529 -63.04 -312.92 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -63.04 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1QWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QWJ-query.scw PDB file : Tito_Scwrl_1QWJ.pdb: