Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGVRGN---GVIR----KLA-APFK-ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVAGFT-----N------------AQLSRV---AK----------------VVCEAFPNTFP--A-S--EKVVTTGNPVRREITDILSPKWRYDEREQAGKPLNILIVGGSLGAK--ALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 2IYA Chain:A ((11-417)) -------TPRHISFFNIPGHGHVNPSLGIVQELVARGHRVSYAITDEFA--AQVKAAGATPVVYDSILPKESNPEESWPEDQESAMGLFLDEAVRVLPQLEDAYADDRPDLIVYDIAS--WPAPVLGRKWDIPFVQLSPTFVAYEGFEEDVPAVQDPTAEDGLVRFFTRLSAFLEEHGVDTPATEFLIAPNRCIVALPRTFQIKGDTVGDNYTFVGPTYGDRSHQ-------GTWEGPGDGRPVLLIALGSAFTDHLDFYRTCLSAVDGL--DWHVVLSVGRFVDPADL---GEVPPN--VEVHQWVPQL-DILTKASAFITHAGMGSTMEALSNAVPMVAVPQIA----EQTMNAERIVELGLGRHIPRDQVTAEKLREAVLAVASDPGVAERLAAVRQEIREAGGARAAADILE--

General information: TITO was launched using: RESULT: Template: 2IYA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1796 -70726 -39.38 -212.39 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -39.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_2IYA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IYA-query.scw PDB file : Tito_Scwrl_2IYA.pdb: