Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISTHILDTNLGKPAAQVEVKLFHTA-THELIATAITNQDGRVIDFGI-KNLGKDAYQLEFEVAPYFSSQQIKTFFPRVCIQFFIEDTNQHYHIPLLISPFAYSTYRGS 2GPZ Chain:A ((2-111)) ILSVHILDQQTGKPAPGVEVVLEQKKDGWTQLNTGHTDQDGRIKALWPEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHISKTNEHYHVPLLLSQYGYSTYRGS

General information: TITO was launched using: RESULT: Template: 2GPZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 481 -25533 -53.08 -238.63 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -53.08 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_2GPZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GPZ-query.scw PDB file : Tito_Scwrl_2GPZ.pdb: