Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNISYELEK--------KIVNLNALYNEEE--AELSLRLSQNIRKNQQDGYVYSLISRGFEIKGLKLWLVDKNIDQLKQWFYVSSVLRAESCKYSSGYTLSTPDEFIFPLLSDNTEIINKFAYLDTVNLLWGEFEPSYQEAKFKPSKDDPRFRTHALQAVLRNDWEDYKKIKEVANQKINKLREVVQFEFGIYDAIYEKDKDKIIEIVQTLLNPRVHKTYNKDFGEEFNGEIWSHHPVMFTKLA----WMNGLEIEIDNPLVPMELMPIKPLEHYDYHYDFLDPNWKPKSLFEKLFGKKRNK 3BF8 Chain:A ((1-255)) MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN-------IIQVDVRNHGLSP-----REPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALAPDRIDKLVAIDIAPVDY--------------HVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDH--------------PALFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHW-VHAE------KPDAVLRAIRRYLNDH

General information: TITO was launched using: RESULT: Template: 3BF8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1325 52456 39.59 217.66 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 39.59 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_3BF8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BF8-query.scw PDB file : Tito_Scwrl_3BF8.pdb: