Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSFAGHIIVGIDA--MNGMVATDGWANVTDV-KATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRNLK--GQLGILGAITGRAIYEGTLNLREAQLLLDENRL 2Y85 Chain:A ((3-238)) LILLPAVDVVEGRAVR---------TEYGS-AVDAALGWQRDGAEWIHLVDLDAAFGRGSNHELLAEVVGKL--DVQVELSGGIRDDESLAAALATGCARVNVGTAALENPQWCARVIGEHGDQVAVGLDVQIIDGEHRLRGRGWETDGGDLWDVLERLDSEGCSRFVVTDITKDGTLGGPNLDLLAGVADRTDAPVIASGGVSSLDDLRAIATLTHRGVEGAIVGKALYARRFTLPQA---------

General information: TITO was launched using: RESULT: Template: 2Y85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1271 -101399 -79.78 -456.75 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -79.78 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.713

(partial model without unconserved sides chains): PDB file : Tito_2Y85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y85-query.scw PDB file : Tito_Scwrl_2Y85.pdb: