Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKFRIDLLRHGESQY--SHTLRGHLDDELTAKGWQQMQATIEQVSNQPWDVIVSSSLKRCVCFAEQLAKTAELPLLVNHDLKEMYFGEWEGISTQQIYETSPELLANFWQKPSQYCPPRAETLDQFQTRVLKGFQDLLEHMQKQNLQHALVVTHGGVIKLLACLARQQPLDDLL-KMPAELGKLYSLEFSETDSQLTFKLR 1H2F Chain:A ((1-190)) --ATTLYLTRHGETKWNVERRMQGWQDSPLTEKGRQDAMRLGKRLEAVELAAIYTSTSGRALETAEIVRGGRLIPIYQDERLREIHLGDWEGKTHDEIRQMDPIAFDHFWQAPHLYAPQRGERFCDVQQRALEAVQSIVDRH---EGETVLIVTHGVVLKTLMAAFKDTPLDHLWSPPYMYGTSVTIIEVDGGTF-------

General information: TITO was launched using: RESULT: Template: 1H2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 949 32882 34.65 175.84 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 34.65 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_1H2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H2F-query.scw PDB file : Tito_Scwrl_1H2F.pdb: