Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIYILGSAAGGGCPQWNCNCPNCHGVRTGTINAKVRTQSSIAISENGVDWILLNASPDIRQQLFDFKAAQPARKLRDTGITNVILMDSQLDHTTGLLTLRE-----------GCPMNVWCTEMVYQDLTTGFPVFNMLKHWNGGLQYHQVDPKQAFIIDGFENLEFLPLIIQSAAPPYSPHRHDPHEGDNIALIIKDHKTQKQLFYAPGLGKIDDQIMQIMQDSDCVMIDGTLWTDDEMQQTGVGKKTGREMGHLYISGEGGSLSYLNQLSTPKKVLIHINNTNPILNEDSAQFAELKANGVEVAFDGMQIEL 1ZKP Chain:D ((25-267)) MKMTVVGFWGGF--PE------------------AGEATSGYLFEHDG-FRLLVDCGSGVLAQLQKY--I------TPSDIDAVVLSHYHHDHVADIGVLQYARLITSATKGQLPELPIYGHTFDENGFHSLTHE--------PHTKGIPYNPEETLQ---IGPFSISFLKTVHPV-------------TCFAMRITAG--NDIVVYSADSSYIPE-FIPFTKDADLFICECNMYAHQE----------AAKAGHMNST---EVASIAKDANVKELLLTHLPHTGNPA-DLVTEAKQIFSGHITLAHSGYVWN-

General information: TITO was launched using: RESULT: Template: 1ZKP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1393 -58702 -42.14 -253.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -42.14 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_1ZKP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZKP-query.scw PDB file : Tito_Scwrl_1ZKP.pdb: