Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVNKTDREWQRELSPEEYRITRQKGTEPAFTGQYWNTKQHGTYVCRCCGAELFSSDAKYDSGCGWPSFFRPLNGSVIDEHEDLTHGMVRTEIVCHDCEAHLGHVFEDGPQPTGLRYCVNSASLQLKTQEKNDEGTYP 3E0O Chain:B ((1-135)) ---MAYNKEEKIKSLNRMQYEVTQNNGTEPPFQNEYWDHKEEGLYVDIVSGKPLFTSKDKFDSQCGWPSFTKPIE-EEVEEKLDTSHGMIRTEVRSRTADSHLGHVFNDGPGPNGLRYCINSAALRFVPKHKLKEEGYE

General information: TITO was launched using: RESULT: Template: 3E0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 653 -29601 -45.33 -219.27 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -45.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_3E0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E0O-query.scw PDB file : Tito_Scwrl_3E0O.pdb: