Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTFTLFFGVP-SGCSFGSIVALEWSGLPYQLCRIEMP-AQCNTEEYRQINPVGETPALLYDNGKVLTESMAILNHIMAKSLNAAFHYEQGTEQFNLFNQKLAFLNTTLFNAFSPHWYSL-EHSSSEGNKQALREYGAVQVKKAFTALENSLTNQTWLMGDQLSFVDGYYAGIARWLKYHNVIDIDQFPNCKRLYEQLQNEAAVKFAHAIEQE 4GF0 Chain:A ((2-205)) --VMLTLYFTPGT-ISVAVAIAIEEAALPYQPVRVDFATAEQTKPDYLAINPKGRVPALRLEDDTILTETGALLDYVAAIAPKAGLVPT-DPTAAAQMRSAMYYLASTMHVAHAHKMRGSRWAKQ-QSSFEDMTAQVPETMAACADFVESDILRGPYVLGEDFSLADPYLFVVCNWLDGDGV-DTAAYPKITTFMQQMTARASVAAVKDK---

General information: TITO was launched using: RESULT: Template: 4GF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 945 -29775 -31.51 -148.13 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -31.51 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_4GF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GF0-query.scw PDB file : Tito_Scwrl_4GF0.pdb: