Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAHLVLASSSPRRRELLQQLGLNFEIYSPDIDESVHEGELVHQYVERLAREKAQAVL-NIFPDSVIIAADTSLGLDGQIIGKPDSKQHAFDIWKQLSGRWHDVFSGICIATQQHILSQVVQTQVEFASLTTQDMEDYWATGEPVGKAGAYAIQGIASQYIPKIQGSYSNVVGLPLYEFSQLFKRVKT 4P0E Chain:B ((2-185)) --SLYLASGSPRRQELLAQLGVTFERIVTGIEAQRQPQESAQQYVVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQVMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASDEPLDKAGAYGIQGLGGCFVRKINGSYHAVVGLPLVETYELLSNFN-

General information: TITO was launched using: RESULT: Template: 4P0E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 977 -56363 -57.69 -307.99 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -57.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4P0E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P0E-query.scw PDB file : Tito_Scwrl_4P0E.pdb: