Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGTPMTVIPTIDIVDALAAEYAAKSPREILELALSQQGEIAISFSG-AEDVVLIDMASRL-GKPFRVFSLDTGRLHP-ETYQFFETVRKHYNINIEICFPDAEAVQSMVNEKGLFSFFKDGHQECCGIRKVQPLRKKLATLDGWITGQRKDQSPGTRTEIP-VVQADAGFSGPGKQLIKYNPLANWSSADVWSYIRMMEIPYNPLHERGFVSIGCEPCTRPVLPNQHEREGRWWWEEATQKECGLHAGNLKK 1GPM Chain:A ((208-404)) ----------------WTPAKIIDDAVARIREQV--GDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDMFGDHFGLNIVHVPAEDRFLSALA-----------------GENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAAKMGLVE--PLKELFKDEVRKIGLELGLPYDMLYRHPFP-----------------------------------------

General information: TITO was launched using: RESULT: Template: 1GPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 713 -28614 -40.13 -167.33 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -40.13 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_1GPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPM-query.scw PDB file : Tito_Scwrl_1GPM.pdb: