Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSANTFPAQIEPFKWAEQGFTWSGQLPLSRFARIAREAVGSIDNQLINID------CKLSMDAYHRIVWLDGHMETKVPMECQRCLEPVEMPIVSDFHIALVDDESLIERLDEDADFIVLGESESSTKGDFDTPASINLLALLEDELLLLMPLSPKHDACEHKHQPTIQNVVEEKRDNPFDVLAGLKGKLNS 4B4P Chain:A ((6-147)) ------SAQVTG-TLLGTGKTNTTQMPA----------LYTWQHQIYNVNFIPSSSGTLTCQAGTILVWKNG-RETQYALECRVSIHH--------------SSGSINESQWGQQSQVGFGTACGNKKCR-----------FTGFEISLRIP----PNAQTYPLSGDLKGSFSLT-NKEVNWSASIYVP---

General information: TITO was launched using: RESULT: Template: 4B4P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 680 25957 38.17 192.27 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 38.17 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.049

(partial model without unconserved sides chains): PDB file : Tito_4B4P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B4P-query.scw PDB file : Tito_Scwrl_4B4P.pdb: