Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSNNLEGMNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILET--AFSRQKEGR---HLITTAIEHPAVLNTMKYL-ETKGFDVTYLPVDE-KGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIKEIGELL---------ADHP-AYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGVN----IPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTLEA---NEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIE-PSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK 3A9Y Chain:B ((18-427)) ------------RKVYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQ---TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVR-GVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVS-ENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSC-GIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQ

General information: TITO was launched using: RESULT: Template: 3A9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2164 30637 14.16 82.80 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : 14.16 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3A9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9Y-query.scw PDB file : Tito_Scwrl_3A9Y.pdb: