Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------KSYKAQKICHFTILKMYKNTWTFVRKDSAPADQTTTIEGIKVFFSKDCIPQHDYRGLY--IEYNGFHHDVSQS 1WI3 Chain:A ((1-71)) GSSGSSGPRSRTKISL-EALGILQ---SFIHDVGLYPD----QEAIHTLSAQLDLPKHTIIKFFQNQRYHVKHSGPSSG

General information: TITO was launched using: RESULT: Template: 1WI3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 3928 22.07 62.35 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 22.07 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_1WI3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WI3-query.scw PDB file : Tito_Scwrl_1WI3.pdb: