Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ECWVELFGPGKVSHGKQ---TGRWNAPYYFNLDPMRLIYLHENCKVNEKHTVLLPDNVKWDVETGMIS--------------------------------- 4Y2H Chain:B ((1-352)) SIAAEREAALERPRRTKRERDQLYYECYSDVSVHEEMIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWK---QALLYLNEPVQVEQDTDVS-----------GEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED

General information: TITO was launched using: RESULT: Template: 4Y2H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 20 748 37.40 14.67 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : 37.40 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.033

(partial model without unconserved sides chains): PDB file : Tito_4Y2H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y2H-query.scw PDB file : Tito_Scwrl_4Y2H.pdb: