Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------WPLTADCNIYPKIGDKSVGWGSGFM--GSVAHVRIEKVDYAVALWK-----RVCKAVKLEELPPGVELFAEPNTLRRNPQPVQRRGLVGREPLGGLELQE 4LL8 Chain:B ((59-171)) MESKLLENLNLLKNENENLNSIFERKNKKLKELEKDYSELSNRYNEQKEKMDQLSKLAKNSSAIEQSCSEKLQNMEVNYNSLLESQNLYRDHYSDEISKLNEKIGLLELELSN

General information: TITO was launched using: RESULT: Template: 4LL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 9 158 17.50 1.69 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 17.50 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.62 QMean score : -0.115

(partial model without unconserved sides chains): PDB file : Tito_4LL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LL8-query.scw PDB file : Tito_Scwrl_4LL8.pdb: