Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------GCQAELLNINQAVVGTGCVQMNYYANIYDSTRRAGYTVNANSNCGLSFGNIQVIPDGYSLRQAGYC---------------------------------- 1V88 Chain:A ((1-130)) GSSGSSGIVMADWLKIRGTLKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCFKLFHPLEQSIWAVKG-PKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKSGPSSG

General information: TITO was launched using: RESULT: Template: 1V88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 156 -790 -5.06 -12.15 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -5.06 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_1V88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V88-query.scw PDB file : Tito_Scwrl_1V88.pdb: