Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDG-GDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR 4YVD Chain:A ((56-352)) ------LYRVISGHLGWVRCIAVEPGN-QWFVTGSADRTIKIWDLASGKL-----KLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVH-TLSGHTNAVATVRCQAAEP--QIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGS-PDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHA----------ESGIFACAFDQSESRLLTAEADKTIKVYRED----

General information: TITO was launched using: RESULT: Template: 4YVD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1845 -143432 -77.74 -499.76 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -77.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4YVD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YVD-query.scw PDB file : Tito_Scwrl_4YVD.pdb: