Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHGFITRRVRPPLSVTHREAERLLE-PKPQGCYLVRFSESA-VTFVLTYRSRTCCRHFLLAQLRDGRHVVLGE----DSAHARLQDLLLHY 1I3Z Chain:A ((5-86)) YYHGCLTK----------RECEALLLKGGVDGNFLIRDSESVPGALCLCVSFKKLVYSYRIFREKHGYYRIETDAHTPRTIFPNLQELVSKY

General information: TITO was launched using: RESULT: Template: 1I3Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 253 -12296 -48.60 -161.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -48.60 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_1I3Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I3Z-query.scw PDB file : Tito_Scwrl_1I3Z.pdb: