Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSWKNWLIKINYCVYKKKRKGRMRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIYFREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFIAADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEGREFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQDEKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKEDNKEYSLKDLITKVSKESDNVAHNLLGYYIS-NQSDATFKSKMSAIMGDDW------------DPKEKLISSKMAGKFMEAIYNQN-------GFVLESLTKTDFDSQRIAKGVS--VKVAHKIGDAD--EFKHDTGVVYAD--SPFILSIFTKNSDY------DTISKIAKDVYEVLK 5EEC Chain:B ((4-264)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LVAEPFAKLEQDF--GGSIGVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKN-------ALVPWSPIS-EKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIG-DTTFRLDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAARLALEGL-

General information: TITO was launched using: RESULT: Template: 5EEC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1187 -16054 -13.52 -70.10 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -13.52 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_5EEC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EEC-query.scw PDB file : Tito_Scwrl_5EEC.pdb: