Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MEQLHFITKLLDIKDPNVQILN---IINKDTHKEIIAKLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGIPTRILLRKRRFKCYHCSKMMVAETSDDVQSYFFSFYYITVLNLALLDSPLLSIYSEKRRKT------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 5MUW Chain:A ((2-276)) PIVVTQAHIDRVGIAADLLDASPVSLQVLGRPTAINTVVIKTYIAAVMELASK-----------------QGGSLAGVDIRPSVLLKD--------TAIFTADVESDVD---------------VLDTGIYSVPGLARKPVTHRWPSEGIYSGVTALMGATGSGKSITLNEKLRPDVLIRWGEVAEAYDELDTAVHISTLDEMLIVCIGLGALGFNVAVDSVRPLLFRLKGAASAGGIVAVFYSLLTDISNLFTQYDCSVVMVVNPMVDAEKIEYVFGQVMASTVGAILCADGNVSRTMFRTNKGRIFN

General information: TITO was launched using: RESULT: Template: 5MUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 223 2452 11.00 27.24 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : 11.00 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_5MUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MUW-query.scw PDB file : Tito_Scwrl_5MUW.pdb: