Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRSGSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKENGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPA-NGEIAVPNDATNESRALYLLQSAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSL--SSVDAAVVNNTFVTEAKLDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESSDGLDQPVW 4NTL Chain:A ((6-247)) --------------------------------DKEITVAVQL--ESSKDILEIAKKEAEKKGYKINIMEVSDNVAYNDAVQHDEADANFAQHQPFMEMFNKEKKADLVAVQPIYYFAGGFYSK------EYQDAKDL-PENAKVGIPSDPTNEGRALAILNANGVIKLKEGVGFNGTVADVVENPKNITFESIDLLNLAKAYDEKDIAMVFCYPAYLEPAGLTTKDAILLEDKE-ASKHYALQVVTRKGE-KDS--EKIKVLKEAMTTKEVAEYIKKNSKGANIPAF

General information: TITO was launched using: RESULT: Template: 4NTL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1243 16331 13.14 68.33 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 13.14 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_4NTL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NTL-query.scw PDB file : Tito_Scwrl_4NTL.pdb: