Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MAKCKK-YEEFGLDS---LLQETRGGRNHAYMTVEEEKAFLARHLKATEAGEFVTIDALFQA-YKKKLGRSY-----TRDAFYQLLKRHGWRNIMPRPEH--PKKADAQ-TI-VASKNKISIQEDKKAL-- 4Q6V Chain:A ((77-211)) YDWNGAMQPLVSKMLQADGVTAGSVLLVDSVNNRTNGSLNANEATETLRNALANN--GKFTLVSVQQLSMAKQQLGLSPQDSLGTRSKAIGIARNVGAQYVLYSSASGNVNAPALQMQLMLVQTGEI-IWSGKGAVQQ

General information: TITO was launched using: RESULT: Template: 4Q6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 419 26644 63.59 237.89 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 63.59 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_4Q6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6V-query.scw PDB file : Tito_Scwrl_4Q6V.pdb: