Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGV--AFEKAQF--GK---HIIVSAIEHPAVKESALWL-KSQGFEVDFAPVDK-KGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAISEFL---------ADKPTISFHVDAVQALAK--IPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPL---LTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQAL---------LN--YPDIFVFSDEENFAPHILTFGIKG--VRGEVIVHAFEDYDIFISTTSAC-SSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR 3A9X Chain:B ((20-427)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQ--TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDL---GVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERL-------PNTCNFSIQGSQLRGYMVLAQCQ--TLLASVGASCHSDHEDRPSPVLLSCGIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQ-----

General information: TITO was launched using: RESULT: Template: 3A9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1983 -13205 -6.66 -36.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -6.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3A9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9X-query.scw PDB file : Tito_Scwrl_3A9X.pdb: