Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEANNTTI--IGYCLRHQEQGK---HIITTAIEHHAVLETIDYLVQHFGFEATIIQPE--NQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILK---QHPAA-------YHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSM----DFDSYLHGGDQEQKKRAGTENLPAIVGMVAA---LKENLEKQEEHFQHVQN-LETAFLAELEGIQYYLNR---GKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG 3A9Y Chain:B ((20-425)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQ---TRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEF-GKRIHLNSRFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSCGIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAV--

General information: TITO was launched using: RESULT: Template: 3A9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2061 -27673 -13.43 -76.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -13.43 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_3A9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9Y-query.scw PDB file : Tito_Scwrl_3A9Y.pdb: