Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDN----YWYTAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAECHKRHVEPFVTLHHFDTPEALH-SNGDFLNRENIEHFVDYAAFCFEEFPE-VNYWTTFNEIGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHALPTKYPLDHENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDLREEDFTALEAAKDLNDFLGINYYMSDWME--AFDGETEIIHNGKGKKGSSKYQ-IKGVGRRVAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEF-VDNTVYDDGRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQ-----ERYPKKSAHWYKKVAETQIID 4ZEN Chain:B ((14-480)) --KPFPPEFLWGAASAAYQVEGAWNEDGKGLSVWDVFAKQPGRTFKGTNGDVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGNGAVNEKGLDFYDRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVKYWVTLNEQNIFISFGYRLGLHPPGVK-DMKRMYEANHIANLANAKVIQSFRHYVPDGKIGPSFAYSPMYPYD-SRPENVLAFENAEEFQNHWWMDVYAWGMYP----QAAWNYLESQGLEPTVAPGDWELLQAAKP--DFMGVNYYQTTTVEHNPPDGVGEGVMNTTGKKGTSTSSGIPGLFKTVRNPHVDTTNWDWAIDPVGLRIGLRRIANRYQ--LPILITENGLGEFDTLEPGDIVNDDYRIDYLRRHVQEIQRAITDGVDVLGYCAWSFTDLLSWLNGYQKRYGFVYVNRDDESEKDLRRIKKKSFYWYQRVIET----

General information: TITO was launched using: RESULT: Template: 4ZEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2637 44599 16.91 98.67 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : 16.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4ZEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZEN-query.scw PDB file : Tito_Scwrl_4ZEN.pdb: