Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTN---------KQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI--KTKFDKIAPILQTIHTSAKELRGEF-APFEEIKKYESLIEEQIP--------YANY--ESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQ------GRLYLIDWEYSSMNDPMWDLAALFLESEF------------------TSQEEETFLSHYESDQT--------------P-VSHEKIAIYKILQDTIWSLWTVYKEEQGE---DFGDYGVNRYQRAVKGLASYGGSDEK 5FTG Chain:A ((32-377)) ------------------FHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------EAMVLESVMFAILAERSLGPKLYGIF--PQGRLEQFIPSR-RLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLG-----

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 914 43609 47.71 169.68 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 47.71 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FTG-query.scw PDB file : Tito_Scwrl_5FTG.pdb: