Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNA-REIEVGLLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI 2ZDG Chain:C ((3-317)) ----VLLIAGGVSPEHEVSLLSAEGVLRHIP---FPTDLAVIAQDGRWLLGEKALTA------LEAKAAPEGEHPFPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEPPVVPFDPPFF-------VKPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGEASPVGEVRYEAPFYDYETKYTPGRAELLIPAPLDPGTQETVQELALKAYKVLGVRGMARVDFFLAE-GELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELA------------

General information: TITO was launched using: RESULT: Template: 2ZDG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1816 -77158 -42.49 -245.72 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -42.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_2ZDG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZDG-query.scw PDB file : Tito_Scwrl_2ZDG.pdb: