Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKIRQSILKISAPVGLKIVFFSVKRFVEVLNS-VPIKKRTMLIYTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL 1NRZ Chain:B ((1-157)) MQITLARIDDRLIHGQVTTVWSKVANAQRIIICNDDVFNDEVRRTLLRQAAPPGMKVNVVSLEKAVAVYHNPQYQDETVFYLFTNPHDVLTMVRQGVQIATLNIGGMAWRPGKKQLTKAVSLDPQDIQAFRELDKLGVKLDLRVVASDPSVNILDKI

General information: TITO was launched using: RESULT: Template: 1NRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 767 -63372 -82.62 -406.23 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -82.62 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_1NRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NRZ-query.scw PDB file : Tito_Scwrl_1NRZ.pdb: