Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSMRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFDQLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKKKKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHSSSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTLSPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN 2BNM Chain:A ((5-74)) --KTASTGFAELLKDRREQVKMDHAALASLLGETPETVAAWENGEGGE-LT----LTQLGRIAHVLGTSIGALTPPA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2BNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 231 -24480 -105.97 -349.71 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -105.97 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_2BNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BNM-query.scw PDB file : Tito_Scwrl_2BNM.pdb: